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Thank you for your reply. I'm interested in the kits which can isolate mitchondria. So Cell Fractionation Kit Standard (ab109719), Mitochondria/Cytosol Fractionation Kit (ab65320), Mitochondria Isolation Kit for Cultured Cells (ab110170) might fit my request. However, as you see, my focus is the mRNAs attached on mitochondria outer membrane, I'm not sure whether these kits can work.
Asked on Dec 30 2013
ab109719 is not suitable for this purpose, this kit is based on detergent fractionation of cellular proteins into three fractions. RNA distribution was not tested with this kit.
Mitochondrial isolation kits (ab110168 – ab110171) are designed to isolate whole mitochondrial organelle, as such these kits are suitable to isolate mRNA that is attached to/ within this compartment. I would recommend to supplement all the buffers of the Mitochondrial Isolation Kit with RNAse inhibitors. We do not have any data regarding the yield of mRNA. The reference below maybe useful reading:
Attardi G, Montoya J.
Methods Enzymol. 1983;97:435-69. No abstract available.
Please let me know if you have any further questions!
Heather AllenAbcam Scientific Support
Answered on Dec 30 2013