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Can this kit ab110170 be used for scale up preps of mitochondria?
Asked on Feb 27 2014
The procedure can accomodate approximately 20 benchtop mitochondria isolations, assuming about 4x10^7 cells per isolation are used. These amounts can be scaled up or down accordingly, as long as the cells are resuspended to 5 mg/ml in Reagent A, and the same volume of Reagent B is also used as indicated in the protocol. Then, you can adjust the amount of reagent C you resuspend the mitochondria in based on your desired end concentration of mitochondria.
Kevin HansonAbcam Scientific Support
Answered on Feb 27 2014