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In the protocol P.6, pestle B is suggested to use for rupturing the cells sample. Why pestle B is preferred? Is it fine to use tight pestle A instead?
Asked on Mar 20 2014
Large clearance pestle (A)[clearance 0.0030-0.0050 in] is used for the initial sample reduction, such as tissue prep;
Small clearance pestle (B) [0.0005-0.0025 in] is used to form the final homogenate.
When prepare cell homogenate, we recommend to use pestle B directly to gain a fine final prep.
Stanley LeungAbcam Scientific Support
Answered on Mar 20 2014