Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)

Submit an Abreview Q&A (25)Specific References (13)

Overview

  • Product name
    Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
    See all Mitochondria Isolation kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

     

     

Properties

Images

  • Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
    Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
    Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.

Protocols

References

This product has been referenced in:
  • Paredes F  et al. Poldip2 is an oxygen-sensitive protein that controls PDH and aKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer. Proc Natl Acad Sci U S A 115:1789-1794 (2018). Read more (PubMed: 29434038) »
  • Uchihara Y  et al. Taxodione induces apoptosis in BCR-ABL-positive cells through ROS generation. Biochem Pharmacol 154:357-372 (2018). Read more (PubMed: 29859988) »

See all 13 Publications for this product

Publishing research using ab110171? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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25 reviews or Q&A

10
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I am using Mitochondria isolation kit for cultured cells (MS853).
I want to try immuneprecipitation (IP) assay from mitochondrial fraction.
I assume this kit can isolate intact mitochondria with complete structure.
I'm wondering whethe...

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1. Yes, the kit isolates intact mitochondria
2. We recommend lysing the mitochondria for IP using lauryl maltoside:
10% lauryl maltoside: ab109857, https://www.abcam.com/10-lauryl-maltoside-solution-ab109857.html
15% lauryl maltoside: ab109...

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Customer kindly contacted us wondering if Reagent C could be frozen after adding protease inhibitors for longer lifetime.

Reagent C of ab110171 can be frozen. This may extend the shelf-life of the reagent, particularly if it has been opened and a bacterial growth is of concern. Since we do not provide the protease inhibitors, we do not know how stable the protease inhibit...

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Thanks for this thorough answer. Does reagent A contain any protein e.g. BSA?
Regards,

Thank you for contacting me. You are welcome with any questions.

I double checked with the laboratory. Reagent A contains no protein. It is a buffered solution containing sucrose and optimised detergents. Regrettably the exact contents it prop...

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Dear technical team,

Our customer is going to purchase ab110171 and would like to know information of homogenizer such as material, shape (could you give me picture?), http://endic.naver.com/enkrEntry.nhn?entryId=a633cfcee8eb4eaa835c11c2a9b8...

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Thank you for contacting us.

The homogenizer is borosilicate glass and is reusable. Below is a link to a full description and picture:


https://us.vwr.com/store/catalog/product.jsp?catalog_number=KT885300-0002


...

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Customer kindly contacted us inquiring about sample preparation and storage.

Thank you for contacting us.

Samples may be prepared as purified mitochondria, crudemitochondria. In most cases whole tissue or cell lysates aresuitable but these may require some sample optimization. Mitochondria samples can be prepared bys...

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I'm looking for a lysis buffer condition that will solubilize plasma membrane but leave mitochondria intact - any suggestions? With the buffer that you recommend, what happens to nuclei?

Thanks for your enquiry.

We have two kits that are designed to isolate mitochondria:

1. ab110168 - Mitochondrial isolation kit for tissues
2. ab110171 - Mitochondrial isolation kit for cultured cells

You can check t...

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Hi there,

I have a customer who has been experiencing difficulties with the Mitochondrial isolation kit (ab110171). She purchased ab110170 and ab110171 in August 2011. Please see the issue below, and attached technical questionnaire

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Thank you for contacting us and reporting the problems this customer has been having with the Mitochondria Isolation Kit for Cultured Cells kits (ab110170 and ab110171).

As mentioned, I have consulted with the lab in MitoSciences to get their...

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Can you tell me more about the Dounce homogenizer in this kit?

Thank you for your enquiry.

The Dounce Homogenizer in this kit is made of glass and includes 2 pestles, and one 2ml column. I have included an image for your reference.

If there is anything else that I can help you with please do ...

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I would like to run the isolated mitochondria on a Western blot. In figure 2 on your datasheet, you show Western blot results with the mitochondrial fraction. Could you tell me how that fraction was prepared for Western blot (i.e. were lysis buffer and...

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Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding ab110171.

The lab used the following procedure to run the mitochondrial fraction on a Western blot:

1. Measure the protein con...

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I would like to measure complex I and III activity before and after a drug treatment which will take 6 hours.How long will the mitochondria be viable/stable after purification with this kit?

If you are planning to use our activity kits to measure complex I activity,there is no needto keep the mitochondria viable. Whole mito preparations may be frozen at -80C and thawed at a later stage for enzyme activity. Howeverfor respiration experiment...

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