Product nameMitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
See all Mitochondria Isolation kits
Species reactivityReacts with: Other species, Mammals
ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml
Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
This product has been referenced in:
- Paredes F et al. Poldip2 is an oxygen-sensitive protein that controls PDH and aKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer. Proc Natl Acad Sci U S A 115:1789-1794 (2018). Read more (PubMed: 29434038) »
- Uchihara Y et al. Taxodione induces apoptosis in BCR-ABL-positive cells through ROS generation. Biochem Pharmacol 154:357-372 (2018). Read more (PubMed: 29859988) »