Key features and details
- Assay type: Quantitative
Product nameMitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
See all Mitochondria Isolation kits
Species reactivityReacts with: Mammals, Other species
ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
ab110171 has been referenced in 13 publications.
- Paredes F et al. Poldip2 is an oxygen-sensitive protein that controls PDH and aKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer. Proc Natl Acad Sci U S A 115:1789-1794 (2018). PubMed: 29434038
- Uchihara Y et al. Taxodione induces apoptosis in BCR-ABL-positive cells through ROS generation. Biochem Pharmacol 154:357-372 (2018). PubMed: 29859988
- Gómez-Serrano M et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . PubMed: 28064117
- Wang K et al. TNF-a promotes extracellular vesicle release in mouse astrocytes through glutaminase. J Neuroinflammation 14:87 (2017). PubMed: 28427419
- Seillier M et al. Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1. EMBO Mol Med 7:802-18 (2015). PubMed: 25828351