Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
See all Mitochondria Isolation kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Associated products
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Related Products
Images
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Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
References (13)
ab110171 has been referenced in 13 publications.
- Paredes F et al. Poldip2 is an oxygen-sensitive protein that controls PDH and aKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer. Proc Natl Acad Sci U S A 115:1789-1794 (2018). PubMed: 29434038
- Uchihara Y et al. Taxodione induces apoptosis in BCR-ABL-positive cells through ROS generation. Biochem Pharmacol 154:357-372 (2018). PubMed: 29859988
- Gómez-Serrano M et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . PubMed: 28064117
- Wang K et al. TNF-a promotes extracellular vesicle release in mouse astrocytes through glutaminase. J Neuroinflammation 14:87 (2017). PubMed: 28427419
- Seillier M et al. Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1. EMBO Mol Med 7:802-18 (2015). PubMed: 25828351