Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
See all Mitochondria Isolation kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals -
Product overview
ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Associated products
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Related Products
Images
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Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab110171 has been referenced in 24 publications.
- Johnson Z et al. Systematic evaluation of parameters in RNA bisulfite sequencing data generation and analysis. NAR Genom Bioinform 4:lqac045 (2022). PubMed: 35669236
- Xiao W et al. TIMP-1 Dependent Modulation of Metabolic Profiles Impacts Chemoresistance in NSCLC. Cells 11:N/A (2022). PubMed: 36230997
- Yang M et al. Deletion of the E3 ubiquitin ligase, Parkin, exacerbates chronic alcohol intake-induced cardiomyopathy through an Ambra1-dependent mechanism. Br J Pharmacol 178:964-982 (2021). PubMed: 33300167
- Yoshida J et al. Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment. iScience 24:103497 (2021). PubMed: 34934919
- Chen Q et al. BRCA1 Deficiency Impairs Mitophagy and Promotes Inflammasome Activation and Mammary Tumor Metastasis. Adv Sci (Weinh) 7:1903616 (2020). PubMed: 32195105