Key features and details
- Assay type: Quantitative
- Assay time: 1 hr
- Sample type: Tissue
Product nameMitochondria Isolation Kit for Tissue
See all Mitochondria Isolation kits
Assay time1h 00m
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze
Tested tissues: brain, liver and heart
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 kit Isolation Buffer 1 x 100ml Wash Buffer 1 x 30ml
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.
ab110168 has been referenced in 45 publications.
- Louwagie EJ et al. Mitochondrial Transfer Improves Cardiomyocyte Bioenergetics and Viability in Male Rats Exposed to Pregestational Diabetes. Int J Mol Sci 22:N/A (2021). PubMed: 33673574
- Huang LS et al. mtDNA Activates cGAS Signaling and Suppresses the YAP-Mediated Endothelial Cell Proliferation Program to Promote Inflammatory Injury. Immunity 52:475-486.e5 (2020). PubMed: 32164878
- Wen JJ et al. Burn-Induced Cardiac Mitochondrial Dysfunction via Interruption of the PDE5A-cGMP-PKG Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32231130
- Tang TT et al. Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI. Sci Adv 6:eaaz0748 (2020). PubMed: 32851154
- Mu J et al. BRD4 inhibition by JQ1 prevents high-fat diet-induced diabetic cardiomyopathy by activating PINK1/Parkin-mediated mitophagy in vivo. J Mol Cell Cardiol 149:1-14 (2020). PubMed: 32941882