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    mitochondria-isolation-kit-for-tissue-ab110168.pdf

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Mitochondria Isolation Kit for Tissue (ab110168)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2)Q&A (40)References (45)

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Western blot - Mitochondria Isolation Kit for Tissue (ab110168)

    Key features and details

    • Assay time: 1 hr
    • Sample type: Tissue

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    Overview

    • Product name

      Mitochondria Isolation Kit for Tissue
      See all Mitochondria Isolation kits
    • Sample type

      Tissue
    • Assay time

      1h 00m
    • Product overview

      ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.


      Mitochondrial isolation protocol summary:
      - suspend minced tissue in isolation buffer
      - homogenize using dounce homogenizer
      - spin at 1,000 g for 10 min and retain supernatant
      - spin at 12,000 g for 15 min and retain pellet
      - resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
      - resuspend pellet in isolation buffer and analyze or freeze

    • Notes

      Tested tissues: brain, liver and heart

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 kit
      Isolation Buffer 1 x 100ml
      Wash Buffer 1 x 30ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Molecular Biology Kits
      • Cell Fractionation Kits
      • Kits/ Lysates/ Other
      • Kits
      • Extraction Kit
      • Mitochondria
    • Alternative names

      • mitochondria purification
      • mitochondrial isolation
      • mitochondrial purification

    Associated products

    • Related Products

      • Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169)
      • Mitochondria Isolation Kit for Cultured Cells (ab110170)
      • Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
      • Protease Inhibitor Cocktail (EDTA-free) (ab201111)

    Images

    • Western blot - Mitochondria Isolation Kit for Tissue (ab110168)
      Western blot - Mitochondria Isolation Kit for Tissue (ab110168)

      Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (45)

    Publishing research using ab110168? Please let us know so that we can cite the reference in this datasheet.

    ab110168 has been referenced in 45 publications.

    • Louwagie EJ  et al. Mitochondrial Transfer Improves Cardiomyocyte Bioenergetics and Viability in Male Rats Exposed to Pregestational Diabetes. Int J Mol Sci 22:N/A (2021). PubMed: 33673574
    • Huang LS  et al. mtDNA Activates cGAS Signaling and Suppresses the YAP-Mediated Endothelial Cell Proliferation Program to Promote Inflammatory Injury. Immunity 52:475-486.e5 (2020). PubMed: 32164878
    • Wen JJ  et al. Burn-Induced Cardiac Mitochondrial Dysfunction via Interruption of the PDE5A-cGMP-PKG Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32231130
    • Tang TT  et al. Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI. Sci Adv 6:eaaz0748 (2020). PubMed: 32851154
    • Mu J  et al. BRD4 inhibition by JQ1 prevents high-fat diet-induced diabetic cardiomyopathy by activating PINK1/Parkin-mediated mitophagy in vivo. J Mol Cell Cardiol 149:1-14 (2020). PubMed: 32941882
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Works well to isolate mitochondria from frozen mouse kidneys

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    I have used this kit to isolate mitochondria from frozen mouse kidneys. The protocol was simple, easy to follow and produced a good amount of mitochondrial protein lysate. Efficiency of mitochondrial isolation was tested by Western blotting the isolated mitochondrial fraction (resuspended from the pellet) using an OxPhos antibody and comparing with the supernatant. In the blot shown, M = molecular weight marker, 1 and 2 = isolated mitochondrial fraction, 3 and 4 = corresponding supernatants to 1 and 2 respectively.

    Abcam user community

    Verified customer

    Submitted Aug 03 2018

    Works well to isolate mitochondrial and cytosolic fractions from arthropods

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    I have used this kit to isolate mitochondria from Drosophila melanogaster and Hogna lenta. I obtained over 50 μg protein in the mitochondria fraction from a dozen flies and the red eye pigment was removed in the first centrifugation step. I was also pleased to discover that the cytosolic fraction obtained is far superior for use in proteasome activity assays with fluorescent substrates (Suc-LLVY-AMC) than the whole lysate which I had used previously.

    Erin Munkácsy

    Verified customer

    Submitted Aug 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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