Mitochondria Isolation Kit for Tissue (ab110168)
Key features and details
- Assay time: 1 hr
- Sample type: Tissue
Overview
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Product name
Mitochondria Isolation Kit for Tissue
See all Mitochondria Isolation kits -
Sample type
Tissue -
Assay time
1h 00m -
Product overview
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze -
Notes
Tested tissues: brain, liver and heart
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Isolation Buffer 1 x 100ml Wash Buffer 1 x 30ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Associated products
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Related Products
Images
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Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.
Datasheets and documents
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SDS download
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Datasheet download
References (58)
ab110168 has been referenced in 58 publications.
- Tashiro S et al. Pirfenidone attenuates acetaminophen-induced liver injury via suppressing c-Jun N-terminal kinase phosphorylation. Toxicol Appl Pharmacol 434:115817 (2022). PubMed: 34890640
- Rajab BS et al. Differential remodelling of mitochondrial subpopulations and mitochondrial dysfunction are a feature of early stage diabetes. Sci Rep 12:978 (2022). PubMed: 35046471
- Zhu Y et al. miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1. Oxid Med Cell Longev 2022:3182931 (2022). PubMed: 35126811
- Gomes KP et al. Proteomic Analysis Suggests Altered Mitochondrial Metabolic Profile Associated With Diabetic Cardiomyopathy. Front Cardiovasc Med 9:791700 (2022). PubMed: 35310970
- Czernik M et al. Mitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos. Sci Rep 12:16152 (2022). PubMed: 36167966