Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue Homogenate
Product nameMitochondria Isolation Kit For Tissue and Cultured Cells
See all Mitochondria kits
Sample typeAdherent cells, Suspension cells, Tissue Homogenate
Mitochondria Isolation Kit For Tissue and Cultured Cells (ab288084), previously known as Mammalian Mitochondria Isolation Kit for Tissue and Cultured Cells (Cat# K288-50), offers two options for the simple and rapid isolation of intact mitochondria.
The first option is utilizing a reagent-based method allowing parallel processing of multiple samples.
The second option uses traditional dounce homogenization, which provides better mitochondrial yield.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 50 tests Mitochondria Isolation Buffer NM 2 x 100ml Reagent A Blue cap 1 x 1ml Storage Buffer NM 1 x 25ml
RelevanceMitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
Mitochondria Integrity Test:
Mitochondria (100 µg) were purified from the mouse liver. Purified mitochondria were analyzed for intactness by using JC-1 dye, which tests the electrochemical proton gradient(ΔΨ) of the inner mitochondrial membrane. Purified mitochondria was thawed on ice and incubated with JC-1 dye (1 µg/ml) for 15 minutes at 37°C. The intact purified mitochondria show aggregation of JC-1 dye whose signal can be measured at Ex/Em = 530/590nm. Treatment with Antimycin A (100 µM) dissipates the mitochondrial membrane potential resulting in reduced florescence signal.
ab288084 has not yet been referenced specifically in any publications.