Product nameMitochondria Isolation Kit for Tissue (with Dounce Homogenizer)
See all Mitochondria Isolation kits
ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Tested tissues: brain, liver and heart
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Isolation Buffer 1 x 100ml Wash Buffer 1 x 30ml
Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4
This product has been referenced in:
- Sun J et al. Anthocyanins isolated from blueberry ameliorates CCl4 induced liver fibrosis by modulation of oxidative stress, inflammation and stellate cell activation in mice. Food Chem Toxicol 120:491-499 (2018). Read more (PubMed: 30056145) »
- Villeneuve LM et al. Proteomic and functional data sets on synaptic mitochondria from rats with genetic ablation of Parkin. Data Brief 20:568-572 (2018). Read more (PubMed: 30197913) »