Overview

  • Product name
    Mitochondria/Cytosol Fractionation Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    4h 00m
  • Product overview

    Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
    Visit our FAQs page for tips and troubleshooting.

Properties

Protocols

References

This product has been referenced in:
  • Yang M  et al. TFAM is a novel mediator of immunogenic cancer cell death. Oncoimmunology 7:e1431086 (2018). Read more (PubMed: 29872558) »
  • Jana S  et al. Reciprocal amplification of caspase-3 activity by nuclear export of a putative human RNA-modifying protein, PUS10 during TRAIL-induced apoptosis. Cell Death Dis 8:e3093 (2017). Read more (PubMed: 28981101) »

See all 17 Publications for this product

Publishing research using ab65320? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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16 reviews or Q&A

10
Votes: Highest First



Inquiry: Hello, I would like to know if I can store the cytosolic and mitochondrial buffers for months in the freezer (-20 C) either as a whole or in aliquots and if not then why please. Thanks

Thank you for contacting us.



We do not recommend freezing the buffers after first thaw for best results. Some components can precipitate out upon freezing.




Thank you for your reply. I'm interested in the kits which can isolate mitchondria. So Cell Fractionation Kit Standard (ab109719), Mitochondria/Cytosol Fractionation Kit (ab65320), Mitochondria Isolation Kit for Cultured Cells (ab110170) mig...

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ab109719 is not suitable for this purpose, this kit is based on detergent fractionation of cellular proteins into three fractions. RNA distribution was not tested with this kit.



Mitochondrial isolation kits (ab110168 – ab1101...

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Inquiry: According to the Protocol Booklet [https://www.abcam.com/ps/products/65/ab65320/documents/ab65320%20Mitochondria%20Cytosol%20Fractionation%20Kit%20%28Website%29.pdf], this product is to be used with cells. On the other hand, on the website [ht...

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I have been in contact with the lab who have confirmed that fresh tissue can be used with this kit. For this sample type, you need to isolate out the cells from the tissues by enzymatic breakdown and follow the protocol mentioned on the datasheet ...

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Thank you for your detailed information:
According to your reply "The supernatant after centrifugation will be mostly cell debris including parts of the membrane. "
Another protein of interest for us is " EP4 receptor" ab4...

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Thank you for your reply.

You would probably be best suited using a kit that is able to isolate the membrane fraction completely if you are looking at a membrane protein like Prostaglandin E Receptor EP4. You may want to use a kit like our ab...

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We are interested to purchase the ab65320 from your company. There are couple questions I have regarding the protocol:
Just a little background before start of my question: Our lab research focus is primarily on human skeletal muscle biopsy sample...

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Thank you for contacting us.

The supernatant after centrifugation will be mostly cell debris including parts of the membrane. This kit is sourced from an outside lab and they consider the components of the buffers to be proprietary so we do ...

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I would like to use this kit for mouse brain tissue.  The instructions are for cells.  Can you tell me how to modify the assay protocol for brain tissue?  For example, how much cytosolic buffer per brain weight, as well as mitochondria extraction buffe...

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Thank you for contacting us.

I have been in contact with the lab regarding your inquiry. For each 10 mg of tissue, use 1 ml of the 1X Cytosolic Extraction Buffer containing DTT and Protease Inhibitors for homogenization. Follow the protoco...

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Dear technical team,

Our customer would like to know if these kits are available to mouse sample.
Please let me know.

Best regards

Thank you for your enquiry and your interest in our products.

I can confirm that both kits can be used for mammalian cells (including mouse samples).

If you need any further assistance in the future, please do not hesitate to contac...

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Inquiry: Dear, I'm writing to you to have an information. I would like order a kit for cytosol and Mitocondrial extraction. I saw your kit: ab109719 and ab65320. Could you, please, explain to me the differences between the kits. I have to extract the f...

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Thank you for your enquiry and interest in our products.

https://www.abcam.com/ab109719 - Cell Fractionation Kit Standard: allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear fractions from cultured cells. Un...

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We recently purchased Mitochondria/CytosolFractionation Kit(ab65320) from Abcam. we do not haveDounce tissue grinder, Can we use sonicator toHomogenize cells after addition of1X Cytosol Extraction Buffer Mix.

please suggest what can i do .


Please do not use a sonicatorin order to destroy your cells, as this will result in a complete lysis of the mitochondria as well. This means you will not be able to analyse the sample.



However, we do have a kit in which we deliv...

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Hallo, I am considering purchasing Ab65320, however I have a couple of questions: my aim is to run WB on total cytoplasmic extract as well as mitochondria free cytoplasmic extract on primary macrophages. I would like to know whether a polytron can repl...

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Unfortunately we do not have any data regarding the use of the kit ab65320with samples homogenised with a polytron. We recommend the use of a Dounce homogenizer. Excessive homogenization should be avoided, as it can cause damage to the mitochondri...

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1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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