• Product name
    Mitochondria/Cytosol Fractionation Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
  • Assay time
    4h 00m
  • Product overview

    Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
    Visit our FAQs page for tips and troubleshooting.




This product has been referenced in:
  • Yang M  et al. TFAM is a novel mediator of immunogenic cancer cell death. Oncoimmunology 7:e1431086 (2018). Read more (PubMed: 29872558) »
  • Jana S  et al. Reciprocal amplification of caspase-3 activity by nuclear export of a putative human RNA-modifying protein, PUS10 during TRAIL-induced apoptosis. Cell Death Dis 8:e3093 (2017). Read more (PubMed: 28981101) »

See all 17 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

We do not recommend freezing the buffers after first thaw for best results. Some components can precipitate out upon freezing.

ab109719 is not suitable for this purpose, this kit is based on detergent fractionation of cellular proteins into three fractions. RNA distribution was not tested with this kit.

Mitochondrial isolation kits (ab110168 – ab1101...

Read More

I have been in contact with the lab who have confirmed that fresh tissue can be used with this kit. For this sample type, you need to isolate out the cells from the tissues by enzymatic breakdown and follow the protocol mentioned on the datasheet ...

Read More

Thank you for your reply.

You would probably be best suited using a kit that is able to isolate the membrane fraction completely if you are looking at a membrane protein like Prostaglandin E Receptor EP4. You may want to use a kit like our ab...

Read More

Thank you for contacting us.

The supernatant after centrifugation will be mostly cell debris including parts of the membrane. This kit is sourced from an outside lab and they consider the components of the buffers to be proprietary so we do ...

Read More

Thank you for contacting us.

I have been in contact with the lab regarding your inquiry. For each 10 mg of tissue, use 1 ml of the 1X Cytosolic Extraction Buffer containing DTT and Protease Inhibitors for homogenization. Follow the protoco...

Read More

Thank you for your enquiry and your interest in our products.

I can confirm that both kits can be used for mammalian cells (including mouse samples).

If you need any further assistance in the future, please do not hesitate to contac...

Read More

Thank you for your enquiry and interest in our products.

https://www.abcam.com/ab109719 - Cell Fractionation Kit Standard: allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear fractions from cultured cells. Un...

Read More

Please do not use a sonicatorin order to destroy your cells, as this will result in a complete lysis of the mitochondria as well. This means you will not be able to analyse the sample.

However, we do have a kit in which we deliv...

Read More

Unfortunately we do not have any data regarding the use of the kit ab65320with samples homogenised with a polytron. We recommend the use of a Dounce homogenizer. Excessive homogenization should be avoided, as it can cause damage to the mitochondri...

Read More

1-10 of 16 Abreviews or Q&A


Sign up