Product nameMitochondria/Cytosol Fractionation Kit
Sample typeTissue, Adherent cells, Suspension cells
Assay time4h 00m
Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
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Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 25 tests 5X Cytosol Extraction Buffer 1 x 20ml 1 x 5ml DTT 1 x 110µl 1 x 110µl Mitochondria Extraction Buffer 1 x 10ml 1 x 2.5ml Protease Inhibitor Cocktail 1 vial 1 vial
RelevanceMitochondria/Cytosol Fractionation is the process for effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of mammalian cells.
This product has been referenced in:
- Chang P et al. Valproic Acid and Neural Apoptosis, Inflammation, and Degeneration 30 Days after Traumatic Brain Injury, Hemorrhagic Shock, and Polytrauma in a Swine Model. J Am Coll Surg 228:265-275 (2019). Read more (PubMed: 30639301) »
- Yang M et al. TFAM is a novel mediator of immunogenic cancer cell death. Oncoimmunology 7:e1431086 (2018). Read more (PubMed: 29872558) »