Anti-Mitochondrial dicarboxylate carrier antibody (ab214205)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Mitochondrial dicarboxylate carrier antibody
See all Mitochondrial dicarboxylate carrier primary antibodies -
Description
Rabbit polyclonal to Mitochondrial dicarboxylate carrier -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Mitochondrial dicarboxylate carrier aa 200-250 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:FTHFVASFIAG GCATFLCQPL DVLKTRLMNS KGEYQGVFHC AVETAKLGPL
Database link: Q9UBX3 -
Positive control
- Human gastric carcinoma and kidney tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab214205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Target
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Relevance
The mitochondrial dicarboxylate carrier protein (mDIC) belongs to superfamily of mitochondrial transporters. It has been demonstrated that the expression of mDIC leads to hyperpolarization of the mitochondria, as well as succinate uptake by the mitochondria. It is highly expressed in adipose tissue, where its expression is downregulated by insulin and upregulated by free fatty acids. mDIC is also thought to play a role in glyceroneogenesis. -
Cellular localization
Mitochondrion; mitochondrial inner membrane; multi pass membrane protein. -
Database links
- Entrez Gene: 1468 Human
- Entrez Gene: 27376 Mouse
- Entrez Gene: 170943 Rat
- Omim: 606794 Human
- SwissProt: Q9UBX3 Human
- SwissProt: Q543J6 Mouse
- SwissProt: Q9D817 Mouse
- SwissProt: Q9QZD8 Mouse
see all -
Alternative names
- DIC antibody
- SLC25A10 antibody
- Solute carrier family 25 member 10 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial dicarboxylate carrier antibody (ab214205)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Mitochondrial dicarboxylate carrier with ab214205 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
References
ab214205 has not yet been referenced specifically in any publications.
