Anti-Mitochondrial dicarboxylate carrier antibody (ab214205)
Key features and details
- Rabbit polyclonal to Mitochondrial dicarboxylate carrier
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mitochondrial dicarboxylate carrier antibody
See all Mitochondrial dicarboxylate carrier primary antibodies -
Description
Rabbit polyclonal to Mitochondrial dicarboxylate carrier -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Mitochondrial dicarboxylate carrier aa 200-250 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:FTHFVASFIAG GCATFLCQPL DVLKTRLMNS KGEYQGVFHC AVETAKLGPL
Database link: Q9UBX3 -
Positive control
- Human gastric carcinoma and kidney tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab214205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Target
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Relevance
The mitochondrial dicarboxylate carrier protein (mDIC) belongs to superfamily of mitochondrial transporters. It has been demonstrated that the expression of mDIC leads to hyperpolarization of the mitochondria, as well as succinate uptake by the mitochondria. It is highly expressed in adipose tissue, where its expression is downregulated by insulin and upregulated by free fatty acids. mDIC is also thought to play a role in glyceroneogenesis. -
Cellular localization
Mitochondrion; mitochondrial inner membrane; multi pass membrane protein. -
Database links
- Entrez Gene: 1468 Human
- Entrez Gene: 27376 Mouse
- Entrez Gene: 170943 Rat
- Omim: 606794 Human
- SwissProt: Q9UBX3 Human
- SwissProt: Q543J6 Mouse
- SwissProt: Q9D817 Mouse
- SwissProt: Q9QZD8 Mouse
see all -
Alternative names
- DIC antibody
- SLC25A10 antibody
- Solute carrier family 25 member 10 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial dicarboxylate carrier antibody (ab214205)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Mitochondrial dicarboxylate carrier with ab214205 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
References (0)
ab214205 has not yet been referenced specifically in any publications.