Overview

  • Product name
    Mitochondrial DNA Isolation Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Direct
  • Product overview

    Abcam's Mitochondrial DNA Isolation Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications
    Visit our FAQs page for tips and troubleshooting.


    Please note: One kit is sufficient for 50 samples if Adherent cell or Suspension cells are used and for 25 samples if tissue is used.

  • Notes

    Mitochondria are semi-autonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA.

Properties

Protocols

References

This product has been referenced in:
  • Szczesny B  et al. Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells. Sci Rep 8:914 (2018). Read more (PubMed: 29343810) »
  • Owa C  et al. Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection. PLoS One 13:e0192722 (2018). Read more (PubMed: 29420656) »

See all 6 Publications for this product

Publishing research using ab65321? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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50 reviews or Q&A

10
Votes: Highest First

I have a question regarding the mitochondrial DNA isolation kit ab65321. Is there any RNase added in any of the buffers (I guess it could be a component of the Enzyme Mix)? In case of added RNase, what type of RNase is it?



I can confirm there is RNase contained in the mitochondrial lysis buffer.

This is RnaseA from Bovine pancreas.

One of our costumers is intending to use Mitochrondrial DNA Isolation Kit - ab65321 for Marine Invertebrate and Reptile specimen.

He knows that this kit hasn't been tested for that kind of samples yet. However, he has questions about the kit...

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Thank you for contacting Abcam regarding ab65321.


Regarding this Mitochondrial DNA Isolation Kit:


1 - Expect to recover 5-20ug mtDNA

2- The most important step with this kit is to isolate a pure mitochondrial ...

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Mitochondrial DNA isolation

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Mitochondrial DNA (mtDNA) isolation has been performed using mitochondrial DNA Isolation Kit (Abcam, ab65321) following the manufacturer’s instructions. MtDNA has been isolated by MRC5 fibloblasts in high yield and purity, without contaminations from genomic DNA. 2.22 x 106 cells have been used in each condition, the concentration of the purified mtDNA was then measured using NanoDrop 2000, and graph presentation has been performed using the GraphPad Prism Version 5.01a statistical package (GraphPad Software Inc., USA). A 1% agarose gel stained with EtBr was also run to confirm the correct size (~ 17kB) using a DNA ladder, the purity and the concentration of mtDNA, and it was imaged using Chemidoc MP Imaging System (Biorad, USA).
Personal feedback: Mitochondrial DNA isolation Kit can be safely used to isolate mtDNA in high yield and purity, by using a standard cell number in each condition, the effect of various treatments in the number of mitochondria can be easily measured.
abreview image
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Nov 10 2016

Extraction of mtDNA from whole blood

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
This is a great kit to extract mitochondrial DNA. We just added an extra step of isolating Peripheral Blood Mononuclear Cells (PBMC) from whole blood and applied the abcam protocol (with minor changes, see attached protocol) to isolate mtDNA from PBMC.
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Username

Lina Abi Mosleh

Verified customer

Submitted Apr 20 2016

Does the Enzyme Mix neutralize reactive oxygen species from mitochondria?

No, the Enzyme B Mix or any other component should not neutralize ROS species.

My sample is whole DNA (genomic + mitochondrial). I'd like to potentially use this kit to isolate the mitochondrial DNA, but I see this kit is more designed for earlier on in the process when your sample is tissue or cells. Do you think the kit would w...

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ab65321 is designed to isolate mitochondria first and then lyse the mitochondria to get the mtDNA. Thus, since you have a solution of DNA with genomic and mtDNA mixed in, then this kit is not designed to isolate the mtDNA specifically. One way the lab ...

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To whom it may concern:



I am writing to you to enquire about the suitability of your kit for the extraction of mtDNA from frozen tissue for downstream epigenetic analysis; specifically, if you may be able to tell me if there are any ...

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Thank you for your enquiries regarding ab65321 Mitochondrial DNA Isolation Kit .

I have now obtained some further information from the laboratories.

As I thought, the Enzyme B composition is proprietary. However, I can confirm there ...

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Inquiry: Hi there, I have a question about the Mitochondrial DNA Isolation Kit (ab65321). For my application I need the isolated mtDNA to remain in the circular form - do you know if the mtDNA isolated by the kit remains circular?

I can confirm that the isolated mtDNA in the mitochondria isolated with this kit will be circular and will run at ˜15-20 kbs.

Is it possible to get some information about the composition of the Cytosol Extraction Buffer and the Mitochondrial Lysis Buffer of the Mitochondrial DNA isolation kit (65321)? Thank you very much in advance.

Vielen Dank für Ihre Geduld.

Leider ist diese Information ein Betriebsgeheimnis, doch das Labor ist gerne bereit, auf mehr spezielle Fragen einzugehen, wie zb. pH- Wert einzelner Reagenzien, oder Verwendung nach der Lyse.

Ich...

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I don't have a Dounce homogenizer. Which one do you recommend?

Thank you for contacting us.

We recommend the 2 mL version of this homogenizer:

https://ca.vwr.com/store/catalog/product.jsp?product_id=4830627

I hope this information is helpful to you. Please do not hesitate to contact...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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