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My sample is whole DNA (genomic + mitochondrial). I'd like to potentially use this kit to isolate the mitochondrial DNA, but I see this kit is more designed for earlier on in the process when your sample is tissue or cells. Do you think the kit would work with my sample, and if so, do you have any protocol recommendations?
Asked on Aug 07 2014
ab65321 is designed to isolate mitochondria first and then lyse the mitochondria to get the mtDNA. Thus, since you have a solution of DNA with genomic and mtDNA mixed in, then this kit is not designed to isolate the mtDNA specifically. One way the lab suggested is to run an agarose gel. Genomic DNA will be a smear and mtDNA would be between 16-20Kb and you can cut the gel piece out and purify DNA from this or do electroelution.
However, the best way to ensure mtDNA purity is to first isolate the mitochondria, then proceed with mtDNA extraction.
Kevin HansonAbcam Scientific Support
Answered on Aug 07 2014