Key features and details
- Rabbit polyclonal to Mitochondrial Ferritin
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Mitochondrial Ferritin antibody
See all Mitochondrial Ferritin primary antibodies
DescriptionRabbit polyclonal to Mitochondrial Ferritin
Tested applicationsSuitable for: WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Mitochondrial Ferritin aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q8N4E7
- This antibody gave a positive signal in both human liver and brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab66111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa).|
FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
Tissue specificityDetected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
Sequence similaritiesBelongs to the ferritin family.
Contains 1 ferritin-like diiron domain.
- Information by UniProt
- FerH antibody
- Ferritin antibody
- Ferritin H subunit antibody
All lanes : Anti-Mitochondrial Ferritin antibody (ab66111) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The band observed at 21 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.
IHC-Fr image of Ferritin, mitochondrial staining on mouse skeletal muscle using ab66111 (1: 100). The sections were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The section was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
ICC/IF image of Ferritin, mitochondrial staining on mouse hippocampal culture using ab66111 (1: 100). The cells were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The cells was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
ab66111 has been referenced in 1 publication.
- Gong S et al. Roflumilast restores cAMP/PKA/CREB signaling axis for FtMt-mediated tumor inhibition of ovarian cancer. Oncotarget 8:112341-112353 (2017). PubMed: 29348829