Product nameAnti-Mitochondrial Ferritin antibody
See all Mitochondrial Ferritin primary antibodies
DescriptionRabbit polyclonal to Mitochondrial Ferritin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in both Hela and HEK293 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).|
FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
Tissue specificityDetected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
Sequence similaritiesBelongs to the ferritin family.
Contains 1 ferritin-like diiron domain.
- Information by UniProt
- FerH antibody
- Ferritin antibody
- Ferritin H subunit antibody
All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
The band observed at 22 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.
ab93428 has not yet been referenced specifically in any publications.