Overview

  • Product name

    Anti-Mitochondrial p110 antibody [2G2]
  • Description

    Mouse monoclonal [2G2] to Mitochondrial p110
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length protein (Human).

  • General notes


    p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi’s anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi’s anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.08% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi’s anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi’s anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.
  • Clonality

    Monoclonal
  • Clone number

    2G2
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 2 - 10 µg/ml. Detects a band of approximately 110 kDa.

References

This product has been referenced in:

  • Paulin-Levasseur M  et al. The 2G2 antibody recognizes an acidic 110-kDa human mitochondrial protein. Histochem J 30:617-25 (1998). Read more (PubMed: 9870762) »
See 1 Publication for this product

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