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    mitochondrial-protein-ip-kit-ab239710.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Mitochondrial Protein IP Kit (ab239710)

  • Datasheet
  • SDS
  • Protocol Booklet
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Overview

  • Product name

    Mitochondrial Protein IP Kit
    See all Mitochondria kits
  • Product overview

    Mitochondrial Protein IP Kit (ab239710) is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K285 Mitochondrial Protein IP Kit. K285-50 is the same size as the 50 test size of ab239710.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: IPmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    10% Digitonin 1 x 1ml
    10% n-Dodecyl-beta-D-maltoside 1 x 1ml
    10% Triton X-100 1 x 1ml
    Mitochondria Protein IP Buffer 1 x 50ml
    Protease Inhibitor Cocktail 1 vial
    Wash Buffer (5X) 1 x 50ml
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
  • Relevance

    Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • Cellular localization

    Mitochondrial

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
Notes
IP
Use at an assay dependent concentration.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab239710? Please let us know so that we can cite the reference in this datasheet.

ab239710 has not yet been referenced specifically in any publications.

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