Mitochondrial Protein IP Kit (ab239710)
- Datasheet
- References
- Protocols
Overview
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Product name
Mitochondrial Protein IP Kit -
Product overview
Mitochondrial Protein IP Kit (ab239710) is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.
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Tested applications
Suitable for: IPmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 50 tests 10% Digitonin 1 x 1ml 10% n-Dodecyl-beta-D-maltoside 1 x 1ml 10% Triton X-100 1 x 1ml Mitochondria Protein IP Buffer 1 x 50ml Protease Inhibitor Cocktail 1 vial Wash Buffer (5X) 1 x 50ml -
Research areas
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Relevance
Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales". -
Cellular localization
Mitochondrial
Applications
Our Abpromise guarantee covers the use of ab239710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. |
Protocols
References
ab239710 has not yet been referenced specifically in any publications.
