Overview

  • Product name

    Mitochondrial Protein IP Kit
  • Product overview

    Mitochondrial Protein IP Kit (ab239710) is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.

  • Tested applications

    Suitable for: IPmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    10% Digitonin 1 x 1ml
    10% n-Dodecyl-beta-D-maltoside 1 x 1ml
    10% Triton X-100 1 x 1ml
    Mitochondria Protein IP Buffer 1 x 50ml
    Protease Inhibitor Cocktail 1 vial
    Wash Buffer (5X) 1 x 50ml
  • Research areas

  • Relevance

    Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • Cellular localization

    Mitochondrial

Applications

Our Abpromise guarantee covers the use of ab239710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Protocols

References

ab239710 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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