Anti-PDK1 antibody [4A11] (ab110025)
Key features and details
- Mouse monoclonal [4A11] to PDK1
- Suitable for: WB, ICC/IF, IHC-P, ELISA, Flow Cyt
- Reacts with: Mouse, Rat, Human, Monkey, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-PDK1 antibody [4A11]
See all PDK1 primary antibodies -
Description
Mouse monoclonal [4A11] to PDK1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Monkey, African green monkey -
Immunogen
Recombinant fragment corresponding to Human PDK1 aa 308-436.
Database link: Q15118 -
Positive control
- Hela, Jurkat, HepG2, PC-12, and Cos7 cell lysate, human breast cancer and Human brain tissues, HELA and Lovo cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4A11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).
|
ICC/IF | (1) |
1/200 - 1/1000.
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IHC-P |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa). |
ICC/IF
1/200 - 1/1000. |
IHC-P
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Expressed predominantly in the heart. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 5163 Human
- Entrez Gene: 228026 Mouse
- Entrez Gene: 116551 Rat
- Omim: 602524 Human
- SwissProt: Q15118 Human
- SwissProt: Q8BFP9 Mouse
- SwissProt: Q63065 Rat
- Unigene: 470633 Human
see all -
Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
Images
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Lanes 1-2 : Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 3 : Beta-actin antibody at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : HRP-Goat Anti-Mouse IgG (Fc) at 1/10000 dilution
Predicted band size: 49 kDa12% SDS gel.
Block the membrane for 1 h at room temperature or overnight at 4°C using blocking buffer, 5% milk.
Incubate in ab110025 overnight at 4°C.
Incubation at room temperature for 1 hour with the secondary antibody.
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All lanes : Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : Cos7 cell lysate
Predicted band size: 49 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
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Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).
Protocols
Datasheets and documents
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Datasheet download
References (34)
ab110025 has been referenced in 34 publications.
- Goyal M et al. Blood progenitor redox homeostasis through olfaction-derived systemic GABA in hematopoietic growth control in Drosophila. Development 149:N/A (2022). PubMed: 34850846
- Cenigaonandia-Campillo A et al. Vitamin C activates pyruvate dehydrogenase (PDH) targeting the mitochondrial tricarboxylic acid (TCA) cycle in hypoxic KRAS mutant colon cancer. Theranostics 11:3595-3606 (2021). PubMed: 33664850
- Yao S et al. The oncogenic and prognostic role of PDK1 in the progression and metastasis of ovarian cancer. J Cancer 12:630-643 (2021). PubMed: 33403023
- Lee YG et al. LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival. Oncogenesis 10:18 (2021). PubMed: 33637676
- Xin W et al. lncRNA LA16c-313D11.11 modulates the development of endometrial cancer by binding to and inhibiting microRNA-205-5p function and indirectly increasing PTEN activity. Int J Oncol 57:355-363 (2020). PubMed: 32319598