Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025)


  • Product name
    Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11]
    See all Mitochondrial Pyruvate dehydrogenase kinase 1 primary antibodies
  • Description
    Mouse monoclonal [4A11] to Mitochondrial Pyruvate dehydrogenase kinase 1
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human Mitochondrial Pyruvate dehydrogenase kinase 1.

  • Positive control
    • NIH3T3, Hela, Jurkat, HepG2, PC-12, and Cos7 cell lysate, human breast cancer and Human brain tissues, HELA and Lovo cells



Our Abpromise guarantee covers the use of ab110025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/200 - 1/1000.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Expressed predominantly in the heart.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
    • HGNC:8809 antibody
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
    • PDH kinase 1 antibody
    • Pdk1 antibody
    • PDK1_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 1 antibody
    • Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
    see all


  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : PC-12 cell lysate
    Lane 6 : Cos7 cell lysate

    Predicted band size: 49 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).


This product has been referenced in:
  • Chen GM  et al. microRNA-145-3p inhibits non-small cell lung cancer cell migration and invasion by targeting PDK1 via the mTOR signaling pathway. J Cell Biochem 119:885-895 (2018). Read more (PubMed: 28661070) »
  • Li Y  et al. MicroRNA-210 induces endothelial cell apoptosis by directly targeting PDK1 in the setting of atherosclerosis. Cell Mol Biol Lett 22:3 (2017). WB . Read more (PubMed: 28536634) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Mouse Tissue lysate - other (muscle and liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
30 µg
muscle and liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 30 2015

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Mouse Cell (Liver mouse tissue)
Liver mouse tissue

Dr. Dimitra Kalamida

Verified customer

Submitted Dec 09 2013


Thank you for contacting us.

As always, if we do not advise a certain method on our datasheet, the customer can use the one which they usually use within their lab. This is the case of ab110025.

For ab65979 I would suggest checking the publication which is cited within the application as we added IHC-P due to this application and haven't tested the antibody ourselves. Nonetheless, it is covered by our guarantee.

In the case of ab30782 we suggest a heat mediated antigen retrieval. The image description on the datasheet mentions that citrate buffer was used, but if the customer prefers to use EDTA it should be fine as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from these antibodies.

The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab65979:

1.) HIF alpha is a notoriously difficult target since it is degraded quickly and only really detectable during optimal hypoxia conditions. During this time is localised in the nucleus
Since the blot shows multiple bands that are all smaller in size than HIFa, it is very likely that we see to results of degradation. I therefore suggest to optimise to hypoxia conditions.
I also strongly recommend to use SDS in the lysis buffer to ensure that the nucleus is lysed as well to retrieve the nuclear HIFa. I suggest to use 0.1% SDS. This can be increased if necessary.

2.) To eliminate the possibility that the background is due to the secondary antibody, I suggest to run a no primary control. By omitting the primary antibody one can be sure that all bands seen are background generated by the secondary antibody.

3.) I also strongly suggest to use a positive control. In this case we can recommend rat brain lysate or HeLa cell lysate.


1.) PFKFB4 is a protein that is phophorylated at multiple sites. Phophogroups can add considerable weight to the calculated protein weight and I am confident that the strong band at ca 60kDa is the correct band.

2.) The background can be reduced by using less antibody and less protein loaded on the gel. Any antibody if used to concentrated will produce high background.

3.) I can also recommend to use a positive control. I suggest HepG2 or NIH3T3 cells.

4.) In general I cannot recommend to mix blocking agents. Either use milk or BSA. The blocking agent also can be optimised. The signal strength and the background can be influenced by the choice of blocking agent.


1.) Mitochondrial Pyruvate dehydrogenase kinase 1 gives relatively a weak band in these results. I therefore suggest to use a positive control such as HeLa cells, Jurkat or HepG2 cells.

2.) Since the general background increases strongly after a short exposure of only 5 seconds, I suggest to run a no primary control to exclude that the background is generated by the secondary antibody.

3.) To have the most efficient lysis of the Mitochondrion matrix where Mitochondrial Pyruvate dehydrogenase kinase 1 is localised I recommend to use 0.1% SDS in the lysis buffer.

We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again.

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