Validated using a knockout cell line
Recombinant
RabMAb

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13009] (ab175190)

Overview

  • Product name
    Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13009]
    See all Mitochondrial Pyruvate dehydrogenase kinase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13009] to Mitochondrial Pyruvate dehydrogenase kinase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Mitochondrial Pyruvate dehydrogenase kinase 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15118

  • Positive control
    • LNCaP and Human fetal heart lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
    • Tissue specificity
      Expressed predominantly in the heart.
    • Sequence similarities
      Belongs to the PDK/BCKDK protein kinase family.
      Contains 1 histidine kinase domain.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
      • HGNC:8809 antibody
      • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
      • PDH kinase 1 antibody
      • Pdk1 antibody
      • PDK1_HUMAN antibody
      • Pyruvate dehydrogenase kinase isoform 1 antibody
      • Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)
      Lane 3: Human fetal heart lysate (20 µg)
      Lane 4: LnCaP cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.


      ab175190 was shown to specifically recognize Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab175190 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13009] (ab175190) at 1/1000 dilution

      Lane 1 : LNCaP lysates
      Lane 2 : Human fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    • Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate (lane 1) labeling Mitochondrial Pyruvate dehydrogenase kinase 1 with ab175190 at 1/10 dilution or 1X PBS (lane 2) negative control.  HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    References

    This product has been referenced in:
    • Wang F  et al. Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations. Osteoarthritis Cartilage 24:1113-24 (2016). Read more (PubMed: 26826302) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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