Product nameAnti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13009]
See all Mitochondrial Pyruvate dehydrogenase kinase 1 primary antibodies
DescriptionRabbit monoclonal [EPR13009] to Mitochondrial Pyruvate dehydrogenase kinase 1
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Mitochondrial Pyruvate dehydrogenase kinase 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15118
- LNCaP and Human fetal heart lysate
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab175190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||1/10 - 1/100.|
FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Tissue specificityExpressed predominantly in the heart.
Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)
Lane 3: Human fetal heart lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab175190 was shown to specifically recognize Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab175190 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13009] (ab175190) at 1/1000 dilution
Lane 1 : LNCaP lysates
Lane 2 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate (lane 1) labeling Mitochondrial Pyruvate dehydrogenase kinase 1 with ab175190 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.