Recombinant
RabMAb

Recombinant Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (ab133789)

Overview

  • Product name

    Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)]
    See all Mitochondrial ribosomal protein L11 primary antibodies
  • Description

    Rabbit monoclonal [EPR9111(B)] to Mitochondrial ribosomal protein L11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Mitochondrial ribosomal protein L11 aa 150-250 (C terminal).

  • Positive control

    • HeLa, HepG2, and LNCaP cells. Human kidney tissue, Human colon tissue, HepG2 cells
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

For unpurified format use at 1/250 - 1/500 dilution.

Target

  • Relevance

    Mitochondrial ribosomal protein L11 belongs to the ribosomal protein L11P family.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • 39S ribosomal protein L11, mitochondrial antibody
    • CGI 113 antibody
    • CGI113 antibody
    • L11mt antibody
    • MRP L11 antibody
    see all

Images

  • Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Mitochondrial ribosomal protein L11 with purified ab133789 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • All lanes : Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (ab133789) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Mitochondrial ribosomal protein L11 with purified ab133789 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Mitochondrial ribosomal protein L11 with purified ab133789 at 1:200 dilution (6.2 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Overlay histogram showing HepG2 cells stained with unpurified ab133789 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133789, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • All lanes : Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (ab133789) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : LNCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • Immunofluorescent staining of Mitochondrial ribosomal protein L11 in HEPG2 cells using ab133789 (unpurified) at 1/250 dilution

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Mitochondrial ribosomal protein L11 using ab133789 (unpurified) at 1/250 dilution

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labelling Mitochondrial ribosomal protein L11 using ab133789 (unpurified) at 1/250 dilution

  • Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal colon tissue.

  • Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal heart tissue.

  • Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal lung tissue.

  • Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal stomach tissue.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Ruan N  et al. Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme. Tissue Eng Regen Med 15:173-181 (2018). Read more (PubMed: 30603545) »
See 1 Publication for this product

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