Overview

  • Product name

    Mitochondrial Staining Kit - Blue - Cytopainter
    See all Mitochondria kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Mitochondrial Staining Kit - Blue | Cytopainter (ab219939) uses a proprietary fluorescent blue dye that selectively accumulates in mitochondria in live cells and can be detected at Ex/Em = 360/445 nm (DAPI filter-compatible). The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria, where it can be retained in mitochondria for a long time thanks to its cell-retaining group. The kit can be easily adapted for many different types of fluorescence platforms, such as microplate assays, and flow cytometry.


    Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Platform

    Fluorescence microscope

Properties

Images

  • HeLa cells stained with CytoPainter Mitochondrial Staining Kit - Blue Fluorescence (ab219939) in a 96 well clear-bottom plate.

Protocols

References

ab219939 has not yet been referenced specifically in any publications.

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