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Mitochondrial Staining Kit - NIR Fluorescence - Cytopainter (ab176747)

  • Datasheet
  • SDS
  • Protocol Booklet
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Image of HeLa cells stained with CytoPainter Mitochondrial Staining Kit – NIR Fluorescence (ab176747)
  • Excitation and Emission spectra of CytoPainter Mitochondrial Staning Kit - NIR Fluorescence (ab176747)

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Overview

  • Product name

    Mitochondrial Staining Kit - NIR Fluorescence - Cytopainter
    See all Mitochondria kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (qualitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Mitochondrial Staining Kit - NIR Fluorescence | Cytopainter (ab176747) is designed to label mitochondria in live cells with red fluorescence. The kit uses our proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The red fluorescent mitochondrial stain used in the kit has Ex/Em = 640/660 nm (Cy5 filter-compatible). The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.


    The kit can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating cells.


    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests
    Live Cell Staining Buffer 1 x 50ml
    MitoNIR Indicator 1 x 100µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Mitochondria Staining kits

Associated products

  • Related Products

    • Mitochondrial Staining Reagent - NIR - Cytopainter (ab176833)

Images

  • Image of HeLa cells stained with CytoPainter Mitochondrial Staining Kit – NIR Fluorescence (ab176747)
    Image of HeLa cells stained with CytoPainter Mitochondrial Staining Kit – NIR Fluorescence (ab176747)

    CytoPainter Mitochondrial Staining Kit – NIR Fluorescence (ab176747). HeLa cells stained with Abcam's CytoPainter Mitochondrial Staining Kit – NIR Fluorescence in a Costar black 96-well plate.

  • Excitation and Emission spectra of CytoPainter Mitochondrial Staning Kit - NIR Fluorescence (ab176747)
    Excitation and Emission spectra of CytoPainter Mitochondrial Staning Kit - NIR Fluorescence (ab176747)

     CytoPainter Mitochondrial Staning Kit - NIR Fluorescence (ab176747).  Excitation and Emission spectra.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab176747? Please let us know so that we can cite the reference in this datasheet.

    ab176747 has been referenced in 1 publication.

    • Ben Safta T  et al. Granzyme B-activated p53 interacts with Bcl-2 to promote cytotoxic lymphocyte-mediated apoptosis. J Immunol 194:418-28 (2015). ICC/IF . PubMed: 25404359

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