Mitochondrial Staining Kit - NIR Fluorescence - Cytopainter (ab176747)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameMitochondrial Staining Kit - NIR Fluorescence - Cytopainter
See all Mitochondria kits -
Sample typeAdherent cells, Suspension cells
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Assay typeCell-based (qualitative)
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Species reactivityReacts with: Mammals, Other species
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Product overview
Mitochondrial Staining Kit - NIR Fluorescence | Cytopainter (ab176747) is designed to label mitochondria in live cells with red fluorescence. The kit uses our proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The red fluorescent mitochondrial stain used in the kit has Ex/Em = 640/660 nm (Cy5 filter-compatible). The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.
The kit can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating cells.
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
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Notes
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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Tested applicationsSuitable for: FM, ICC/IFmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 500 tests Live Cell Staining Buffer 1 x 50ml MitoNIR Indicator 1 x 100µl -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Images
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Image of HeLa cells stained with CytoPainter Mitochondrial Staining Kit – NIR Fluorescence (ab176747)CytoPainter Mitochondrial Staining Kit – NIR Fluorescence (ab176747). HeLa cells stained with Abcam's CytoPainter Mitochondrial Staining Kit – NIR Fluorescence in a Costar black 96-well plate.
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Excitation and Emission spectra of CytoPainter Mitochondrial Staning Kit - NIR Fluorescence (ab176747)CytoPainter Mitochondrial Staning Kit - NIR Fluorescence (ab176747). Excitation and Emission spectra.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Ben Safta T et al. Granzyme B-activated p53 interacts with Bcl-2 to promote cytotoxic lymphocyte-mediated apoptosis. J Immunol 194:418-28 (2015). ICC/IF . Read more (PubMed: 25404359) »
