Mitochondrial Staining Kit - Orange Fluorescence - Cytopainter (ab112144)


  • Product name
    Mitochondrial Staining Kit - Orange Fluorescence - Cytopainter
    See all Mitochondria kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
  • Product overview

    Mitochondrial Staining Kit - Orange Fluorescence | Cytopainter (ab112144) is designed to label mitochondria in live cells with orange fluorescence at Ex/Em = 545/575nm (TRITC filter-compatible). This kits uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. ab112144 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry.

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Tested applications
    Suitable for: FM, ICC/IFmore details



Our Abpromise guarantee covers the use of ab112144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • CytoPainter Mitochondrial Staining Kit - Orange Fluorescence (ab112144). Image of U2OS cells stained with ab112144 in a black 96-well plate


ab112144 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We have used this kit for staining mitochondria in living ES cells and found that it woks well.
The product is just easy to use. Note that once you permeabilize the cells before fixation, you will lose signals.

Abcam user community

Verified customer

Submitted Mar 12 2018

J'ai reçu confirmation que les kits de marquage fluorescent des mitochondries CytoPainter :
1) peuvent être utilisés sur des cellules vivantes,
2) ne stoppent pas la division cellulaire,
3) Ne sont à priori pas to...

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Merci de nous avoir contactés.

Le Draq5 se lie à l'ADN et empêche donc malheureusement la division cellulaire.

Un autre produit me semble intéressant: CytoPainter Mitochondrial Staining Kit - Orange Fluoresc...

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