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Mitochondrial Staining Kit - Red Fluorescence - Cytopainter (ab112145)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (3)

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Fluorescence Microscopy- CytoPainter Mitochondrial Staining Kit - Red Fluorescence (ab112145)

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    Overview

    • Product name

      Mitochondrial Staining Kit - Red Fluorescence - Cytopainter
      See all Mitochondria kits
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based
    • Product overview

      Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


      ab112145 is designed to label mitochondria in live cells with red fluorescence. The kit uses our proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The red fluorescent mitochondrial stain used in the kit has Ex/Em = 580/600 nm. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.


      ab112145 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating cells.


      Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide

    • Notes

      Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

    • Platform

      Fluorescence microscope

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 500 tests
      Live Cell Staining Buffer             1 x 50ml
      MitoRed Indicator 1 x 100µl
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Staining Kits
      • Mitochondria Staining kits

    Associated products

      Images

      • Fluorescence Microscopy- CytoPainter Mitochondrial Staining Kit - Red Fluorescence (ab112145)
        Fluorescence Microscopy- CytoPainter Mitochondrial Staining Kit - Red Fluorescence (ab112145)
        Image of U2OS cells stained with ab112145 in a black 96-well plate.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab112145? Please let us know so that we can cite the reference in this datasheet.

      ab112145 has been referenced in 3 publications.

      • Peng S  et al. Achyranthes bidentata polypeptide protects dopaminergic neurons from apoptosis induced by rotenone and 6-hydroxydopamine. Neural Regen Res 13:1981-1987 (2018). PubMed: 30233073
      • Choi JH  et al. Oxyresveratrol Increases Energy Expenditure through Foxo3a-Mediated Ucp1 Induction in High-Fat-Diet-Induced Obese Mice. Int J Mol Sci 20:N/A (2018). PubMed: 30577593
      • Song NJ  et al. Prdm4 induction by the small molecule butein promotes white adipose tissue browning. Nat Chem Biol 12:479-81 (2016). PubMed: 27159578

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      1) Could these products stain the mitochodria after methanol fixed?
      2) Does these products stain Drosophila mitochondria?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 24 2013

      Answer

      1) Most likely not, it is for live cell mitochondria staining.
      2) We don't have experiences on Drosophila mitochondria, it should work theoretically.

      Read More

      尚志 村口

      Abcam Scientific Support

      Answered on Jul 24 2013

      Question

      Draq5 :
      Can it be used on live cells? Does it stop / prevent the cell division?
      Is it toxic? If yes, after how many hours / days the cell die?
      Can it be used in combination with other fluorescent dyes?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 13 2012

      Answer

      J'ai reçu confirmation que les kits de marquage fluorescent des mitochondries CytoPainter :
      1) peuvent être utilisés sur des cellules vivantes,
      2) ne stoppent pas la division cellulaire,
      3) Ne sont à priori pas toxiques,
      4) Peuvent être utilisés avec d'autres marqueurs fluorescents.

      Les lien vers les fiches techniques des kits "CytoPainter Mitochondrial Staining" sont:
      https://www.abcam.com/ab112143 marquage vert (490/520 nm),
      https://www.abcam.com/ab112144 marquage orange (545/575 nm),
      https://www.abcam.com/ab112145 marquage rouge (580/600 nm)

      Le lien vers la page générale des marqueurs CytoPainter est : https://www.abcam.com/cytopainter.

      J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

      Read More

      Abcam Scientific Support

      Answered on Nov 13 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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