Mitochondrial Staining Kit - Red Fluorescence - Cytopainter (ab112145)

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Overview

  • Product name
    Mitochondrial Staining Kit - Red Fluorescence - Cytopainter
    See all Mitochondria kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112145 is designed to label mitochondria in live cells with red fluorescence. The kit uses our proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The red fluorescent mitochondrial stain used in the kit has Ex/Em = 580/600 nm. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.

    ab112145 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating cells.

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Fluorescence Microscopy- CytoPainter Mitochondrial Staining Kit - Red Fluorescence (ab112145)
    Fluorescence Microscopy- CytoPainter Mitochondrial Staining Kit - Red Fluorescence (ab112145)
    Image of U2OS cells stained with ab112145 in a black 96-well plate.

Protocols

References

ab112145 has not yet been referenced specifically in any publications.

Publishing research using ab112145? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

1) Could these products stain the mitochodria after methanol fixed?
2) Does these products stain Drosophila mitochondria?

1) Most likely not, it is for live cell mitochondria staining.
2) We don't have experiences on Drosophila mitochondria, it should work theoretically.

Draq5 :
Can it be used on live cells? Does it stop / prevent the cell division?
Is it toxic? If yes, after how many hours / days the cell die?
Can it be used in combination with other fluorescent dyes?

J'ai reçu confirmation que les kits de marquage fluorescent des mitochondries CytoPainter :
1) peuvent être utilisés sur des cellules vivantes,
2) ne stoppent pas la division cellulaire,
3) Ne sont à priori pas to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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