Overview

  • Product name

    Mitochondrial Staining Reagent - Blue - Cytopainter
    See all Mitochondria kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Mitochondrial Staining Reagent - Blue | Cytopainter (ab219940) is one of a series of fluorogenic probes to label mitochondria of live cells. The MitoBlue Indicator selectively accumulates in mitochondria in live cells and can be easily detected at Ex/Em = 350/490 nm (DAPI-filter compatible). The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria, where it can be retained in mitochondria for a long time thanks to its cell-retaining group.


    Cells stained with MitoBlue Indicator can be further fixed in aldehyde for use in multi-color or long-term staining experiments.


    Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Platform

    Fluorescence microscope

Properties

Images

  • HeLa cells stained with the CytoPainter MitoBlue Indicator Reagent (ab219940) in a 96 well clear-bottom plate.

Protocols

References

ab219940 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Cytopainter-Blue- Rhod-2AM co-staining

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Abreviews
SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Cytopainter-Blue working solution was prepared as per the protocol booklet, in serum free DMEM-F12 media and the cells were treated for 1 hr at 37oC/5% CO2. The stain was gently washed away with PBS 3 times and cells were subsequently treated with 5 μm Rhod-2AM for 30 minutes at 37oC/5% CO2. Following treatment, cells were washed thrice in PBS, fixed in 4% PFA and mounted using Antifade fluorescent mounting media and images were taken using Confocal LSM.

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Verified customer

Submitted Feb 28 2018

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