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    mitochondrial-staining-reagent-blue-cytopainter-ab219940.pdf

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Mitochondrial Staining Reagent - Blue - Cytopainter (ab219940)

  • Datasheet
  • SDS
  • Protocol Booklet
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CytoPainter MitoBlue Indicator Reagent (ab219940)

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    Overview

    • Product name

      Mitochondrial Staining Reagent - Blue - Cytopainter
      See all Mitochondria kits
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Mitochondrial Staining Reagent - Blue | Cytopainter (ab219940) is one of a series of fluorogenic probes to label mitochondria of live cells. The MitoBlue Indicator selectively accumulates in mitochondria in live cells and can be easily detected at Ex/Em = 350/490 nm (DAPI-filter compatible). The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria, where it can be retained in mitochondria for a long time thanks to its cell-retaining group.


      Cells stained with MitoBlue Indicator can be further fixed in aldehyde for use in multi-color or long-term staining experiments.


      Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide

    • Notes

      Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

    • Platform

      Fluorescence microscope

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 500 tests
      MitoBlue Indicator 1 x 500 tests
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Staining Kits
      • Mitochondria Staining kits

    Images

    • CytoPainter MitoBlue Indicator Reagent (ab219940)
      CytoPainter MitoBlue Indicator Reagent (ab219940)

      HeLa cells stained with the CytoPainter MitoBlue Indicator Reagent (ab219940) in a 96 well clear-bottom plate.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab219940? Please let us know so that we can cite the reference in this datasheet.

    ab219940 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Cytopainter-Blue- Rhod-2AM co-staining

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Cytopainter-Blue working solution was prepared as per the protocol booklet, in serum free DMEM-F12 media and the cells were treated for 1 hr at 37oC/5% CO2. The stain was gently washed away with PBS 3 times and cells were subsequently treated with 5 μm Rhod-2AM for 30 minutes at 37oC/5% CO2. Following treatment, cells were washed thrice in PBS, fixed in 4% PFA and mounted using Antifade fluorescent mounting media and images were taken using Confocal LSM.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 28 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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