SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Cytopainter-Blue working solution was prepared as per the protocol booklet, in serum free DMEM-F12 media and the cells were treated for 1 hr at 37oC/5% CO2. The stain was gently washed away with PBS 3 times and cells were subsequently treated with 5 μm Rhod-2AM for 30 minutes at 37oC/5% CO2. Following treatment, cells were washed thrice in PBS, fixed in 4% PFA and mounted using Antifade fluorescent mounting media and images were taken using Confocal LSM.
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Submitted Feb 28 2018