Mitochondrial Staining Reagent - Green - Cytopainter (ab176830)
Overview
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Product name
Mitochondrial Staining Reagent - Green - Cytopainter
See all Mitochondria kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Mitochondrial Staining Reagent - Green | Cytopainter (ab176830) is part of a series of new fluorogenic probes to label mitochondria of live cells. The proprietary mitochondria dye selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency; make it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.
Please note the dye will stain live cells but is not well-retained after fixation.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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Notes
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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Tested applications
Suitable for: FM, Flow Cytmore details -
Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests CytoPainter MitoGreen Indicator Reagent 1 x 500 tests -
Research areas
Associated products
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Corresponding kit
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
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FM
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
Images
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U2OS cells stained with CytoPainter MitoGreen Indicator Reagent (ab176830)
Image of U2OS cells stained with Abcam's CytoPainter MitoGreen Indicator Reagent (ab176830) in a Costar black wall/clear bottom 96-well plate.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab176830 has been referenced in 2 publications.
- Hassing B et al. Phosphatidic acid produced by phospholipase D is required for hyphal cell-cell fusion and fungal-plant symbiosis. Mol Microbiol 113:1101-1121 (2020). PubMed: 32022309
- Huang CC et al. Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells. PLoS One 10:e0128045 (2015). PubMed: 26029922