Mitochondrial Staining Reagent - NIR - Cytopainter (ab176833)

Overview

  • Product name
    Mitochondrial Staining Reagent - NIR - Cytopainter
    See all Mitochondria kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Abcam's Mitochondrial Staining Reagent - NIR | Cytopainter (ab176833) is part of a series of new fluorogenic probes to label mitochondria of live cells. The proprietary mitochondria dye selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency; make it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • HeLa cells mitochondria were stained with Abcam's CytoPainter MitoNIR Indicator Reagent (ab176833), and nuclei were stained with Hoechst 33342 in a Costar black 96-well plate.

Protocols

References

ab176833 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


The mitochondrial membrane potential will not affect the absorbance of this dye, and it is mitochondrial membrane potential independent.

Abreviews
We performed mitochondrial staining on Hydractinia echinata, a marine invertebrate.
The animals were incubates for 1.5 hours in 1:1000 dilution. Animals were then fixed in 4% PFA in PBS and stained with Hoescht at 1:2000 dilution in PBS before being mounted in 90% glycerol.
The picture shows staining in the tentacles(hoescht in blue, mitochondria in red)
Username

Mr. James Gahan

Verified customer

Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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