Mitochondrial Staining Reagent - Orange 405 - CytoPainter  (ab176834)

Overview

  • Product name
    Mitochondrial Staining Reagent - Orange 405 - CytoPainter 
    See all Mitochondria kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Abcam's Mitochondrial Staining Reagent - Orange 405 | CytoPainter (ab176834) is part of a series of new fluorogenic probes to label mitochondria of live cells. The proprietary mitochondria dye selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency; make it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.


    MitoOrange 405 Indicator Reagent is well excited by the violet laser at Ex=405 nm, thus is particularly useful for microscopes and flow cytometers with a violet laser source.

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Image of HeLa cells stained with Abcam's CytoPainter MitoOrange 405 Indicator Reagent (ab176834) in a Costar black wall/clear bottom 96-well plate.

  • Spectrum of CytoPainter MitoOrange 405 Indicator Reagent (ab176834).

Protocols

References

ab176834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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