Overview

  • Product name
    Mitochondrial Staining Reagent - Red - Cytopainter
    See all Mitochondria kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Abcam's Mitochondrial Staining Reagent - Red | Cytopainter (ab176832) is part of a series of new fluorogenic probes to label mitochondria of live cells. The proprietary mitochondria dye selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency; make it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Image of U2OS cells stained with Abcam's CytoPainter MitoRed Indicator Reagent (ab176832) in a Costar black wall/clear bottom 96-well plate.

Protocols

References

ab176832 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Mitochondrial staining followed by PFA fixation

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The protocol was followed for mitochondrial staining of HCT116 cell line. Following incubation of the cells with the mitochondrial staining reagent the cells were fixed with 4% PFA at room temperature for 20 min. Followed by a permiabilization step with 0.1% Triton X-100 for 5 minutes, to confirm the stability of the mitochondrial dye for future use with antibodies staining. In conclusion, the mitochondrial staining reagent is very stable and it could be potentially used with PFA fixation and cells permiabilization, facilitating further immunofluorescence experiments.

Dr. Dimitra Kalamida

Verified customer

Submitted Aug 01 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up