Mitochondrial Superoxide Indicator Assay Kit (Red) (ab228567)

Overview

  • Product name

    Mitochondrial Superoxide Indicator Assay Kit (Red)
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells
  • Assay type

    Cell-based
  • Product overview

    ab228567 Mitochondrial Superoxide Indicator (Red) Assay Kit utilizes a sensitive detection reagent for mitochondrial superoxide that rapidly partitions to the mitochondria where it is oxidized by superoxide and exhibits red fluorescence. The reagent is resistant to oxidation by other ROS systems and is selective only for superoxide.


    This kit may be useful for the investigation of agents that modulate oxidative stress found in various disease states.


    The protocol provides enough reagents to analyze 10 x 96 wells (960 samples) using the methods described.

  • Notes

    Superoxide occurs in mitochondria as a result of incompletely reduced oxygen being produced during oxidative phosphorylation and going on to interact with molecular oxygen and forming superoxide. Increases in cellular superoxide production have been linked to cardiovascular diseases such as hypertension and atherosclerosis and in neurodegenerative disorders such as Parkinson's, Alzheimer's, and amyotrophic lateral sclerosis (ALS).

     

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 kit
    5X Live Cell Imaging Buffer with Hoechst 33342 1 x 20ml
    DMSO 1 x 500µl
    HBSS Buffer 1 x 100ml
    Superoxide Detection Agent 10 x 50µg

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with Mitochondrial superoxide indicator (red).

Protocols

References

ab228567 has not yet been referenced specifically in any publications.

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