Mitochondrial Tracker Green Staining Kit (ab228568)
Overview
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Product name
Mitochondrial Tracker Green Staining Kit -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Assay duration
Multiple steps standard assay -
Product overview
The Mitochondrial Tracker Green Staining Kit (ab228568) uses a green-fluorescent stain that is effectively chemically equivalent to MitoTracker® Green FM. The stain localizes in mitochondria regardless of mitochondrial membrane potential to easily stain and quantify active mitochondria.
This type of staining has been widely used to monitor mitochondrial viability, fragmentation, damage or loss in a variety of pathologies including Alzheimer's disease, Huntington's disease and cardiovascular diseases as well as for cell cycle, apoptosis, mitophagy, chemotaxis and morphogenesis analyses.
Note: The dye will stain live cells but is not well-retained after fixation.
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Notes
The protocol provides enough reagents to analyze 15 x 96 wells (1440 samples).
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Tested applications
Suitable for: ICC/IFmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 1 kit 5X Live Cell Imaging Buffer 1 x 30ml DMSO 1 x 100µl Mitochondrial Tracker Green 1 x 50µg
Applications
Our Abpromise guarantee covers the use of ab228568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. |
Images
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Typical Data
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained using ab228568 and TMRE mitochondrial stain.
References (0)
ab228568 has not yet been referenced specifically in any publications.
