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    mitochondrial-tracker-green-staining-kit-ab228568.pdf

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Mitochondrial Tracker Green Staining Kit (ab228568)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Data

    Overview

    • Product name

      Mitochondrial Tracker Green Staining Kit
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based
    • Assay duration

      Multiple steps standard assay
    • Product overview

      The Mitochondrial Tracker Green Staining Kit (ab228568) uses a green-fluorescent stain that is effectively chemically equivalent to MitoTracker® Green FM. The stain localizes in mitochondria regardless of mitochondrial membrane potential to easily stain and quantify active mitochondria.


      This type of staining has been widely used to monitor mitochondrial viability, fragmentation, damage or loss in a variety of pathologies including Alzheimer's disease, Huntington's disease and cardiovascular diseases as well as for cell cycle, apoptosis, mitophagy, chemotaxis and morphogenesis analyses.


      Note: The dye will stain live cells but is not well-retained after fixation.

    • Notes

      The protocol provides enough reagents to analyze 15 x 96 wells (1440 samples).

    • Tested applications

      Suitable for: ICC/IFmore details

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 kit
      5X Live Cell Imaging Buffer 1 x 30ml
      DMSO 1 x 100µl
      Mitochondrial Tracker Green 1 x 50µg

    Applications

    Our Abpromise guarantee covers the use of ab228568 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use at an assay dependent concentration.

    Images

    • Typical Data
      Typical Data

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained using ab228568 and TMRE mitochondrial stain.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab228568? Please let us know so that we can cite the reference in this datasheet.

    ab228568 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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