Product nameAnti-Mitofilin antibody [2E4AD5] (HRP)
See all Mitofilin primary antibodies
DescriptionMouse monoclonal [2E4AD5] to Mitofilin (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Tissue, cells or virus corresponding to Human Mitofilin. Human heart mitochondria.
- WB: Human heart mitochondria lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab198037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Cell proliferation inducing protein 52 antibody
- Cell proliferation-inducing gene 4/52 protein antibody
- Heart muscle protein antibody
All lanes : Anti-Mitofilin antibody [2E4AD5] (HRP) (ab198037) at 1/5000 dilution
Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198037 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198037 has not yet been referenced specifically in any publications.