Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)

Overview

  • Product name
    Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker
    See all Mitofilin primary antibodies
  • Description
    Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, In-Cell ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Human heart mitochondria.

  • Positive control
    • Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand. 

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

In-Cell ELISA Use a concentration of 4 µg/ml.
IP Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg

    Predicted band size: 84 kDa



    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
  • Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
  • Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).
  • All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)

    Lane 1 : Control neonatal mitochondria from skin fibroblasts
    Lane 2 : Patient 2 (male) mitochondria from skin fibroblasts
    Lane 3 : Patient 1 (female) mitochondria from skin fibroblasts

    Predicted band size: 84 kDa



    Clinical findings for patients 1 & 2 suggest neonatal onset mitochondrial encephalopathy with recurrent bouts of liver disease.

References

This product has been referenced in:
  • Tsai PI  et al. Drosophila MIC60/Mitofilin Conducts Dual Roles in Mitochondrial Motility and Crista Structure. Mol Biol Cell N/A:N/A (2017). WB, IP ; Human . Read more (PubMed: 28904209) »
  • Monterisi S  et al. PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28463107) »

See all 24 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Dec 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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