Overview

  • Product name

    Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker
    See all Mitofilin primary antibodies
  • Description

    Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, In-Cell ELISA, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
  • General notes

    Western blot advice:

    Hydrophobic intrinsic membrane proteins such as core mtDNA-encoded proteins tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

    Product was previously marketed under the MitoSciences sub-brand. 

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab110329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

In-Cell ELISA Use a concentration of 4 µg/ml.
IP Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg

    Predicted band size: 84 kDa



    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
  • Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
  • Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).
  • All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)

    Lane 1 : Control neonatal mitochondria from skin fibroblasts
    Lane 2 : Patient 2 (male) mitochondria from skin fibroblasts
    Lane 3 : Patient 1 (female) mitochondria from skin fibroblasts

    Predicted band size: 84 kDa



    Clinical findings for patients 1 & 2 suggest neonatal onset mitochondrial encephalopathy with recurrent bouts of liver disease.

References

This product has been referenced in:

  • Zhou W  et al. PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction. Hum Mol Genet 28:1100-1116 (2019). Read more (PubMed: 30496485) »
  • Koike S & Jahn R SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. Nat Commun 10:1608 (2019). Read more (PubMed: 30962439) »
See all 31 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T and HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293T and HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 31 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 17 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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