Product nameAnti-Mitofilin antibody
See all Mitofilin primary antibodies
DescriptionRabbit polyclonal to Mitofilin
Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Mitofilin aa 76-215.
Database link: Q16891
- WB: HeLa, HepG2 and MCF7 whole cell lysates. IHC-P: Human glioma cancer and kidney tissues. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
|IP||1/200 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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All lanes : Anti-Mitofilin antibody (ab229653) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Paraffin-embedded human glioma cancer tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mitofilin (green) using ab229653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Mitofilin was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 8 µg ab229653.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in HepG2 whole cell lysate.
Lane 2: ab229653 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 µg.
For western blotting, an HRP-conjugated Protein G antibody was used as the Secondary antibody at 1/2000 dilution.
ab229653 has not yet been referenced specifically in any publications.