Key features and details
- Rabbit polyclonal to Mitofilin
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mitofilin antibody
See all Mitofilin primary antibodies
DescriptionRabbit polyclonal to Mitofilin
Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Mitofilin aa 76-215.
Database link: Q16891
- WB: HeLa, HepG2 and MCF7 whole cell lysates. IHC-P: Human glioma cancer and kidney tissues. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
|IP||1/200 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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All lanes : Anti-Mitofilin antibody (ab229653) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Paraffin-embedded human glioma cancer tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mitofilin (green) using ab229653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Mitofilin was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 8 µg ab229653.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in HepG2 whole cell lysate.
Lane 2: ab229653 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 µg.
For western blotting, an HRP-conjugated Protein G antibody was used as the Secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229653 has not yet been referenced specifically in any publications.