Overview

  • Product name

  • Description

    Rabbit polyclonal to Mitofilin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Mitofilin aa 76-215.
    Database link: Q16891

  • Positive control

    • WB: HeLa, HepG2 and MCF7 whole cell lysates. IHC-P: Human glioma cancer and kidney tissues. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab229653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
IP 1/200 - 1/2000.
ICC/IF 1/50 - 1/200.

Target

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing protein 52 antibody
    • Cell proliferation-inducing gene 4/52 protein antibody
    • Heart muscle protein antibody
    • HMP antibody
    • IMMT antibody
    • IMMT_HUMAN antibody
    • Inner membrane protein mitochondrial antibody
    • Inner mitochrondial membrane antibody
    • MICOS complex subunit MIC60 antibody
    • MINOS2 antibody
    • Mitochondrial inner membrane organizing system 2 antibody
    • Mitochondrial inner membrane protein antibody
    • Mitofilin antibody
    • Motor protein antibody
    • p87 antibody
    • p87/89 antibody
    • p89 antibody
    • pig4 antibody
    • PIG52 antibody
    • Proliferation-inducing gene 4 antibody
    see all

Images

  • All lanes : Anti-Mitofilin antibody (ab229653) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa

  • Paraffin-embedded human glioma cancer tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mitofilin (green) using ab229653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

  • Mitofilin was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 8 µg ab229653. 

    Lane 1: Rabbit monoclonal IgG (1 µg) IP in HepG2 whole cell lysate.

    Lane 2: ab229653 IP in HepG2 whole cell lysate.

    Lane 3: HepG2 whole cell lysate 10 µg.

    For western blotting, an HRP-conjugated Protein G antibody was used as the Secondary antibody at 1/2000 dilution.

References

ab229653 has not yet been referenced specifically in any publications.

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