Product nameAnti-Mitofilin antibody
See all Mitofilin primary antibodies
DescriptionRabbit polyclonal to Mitofilin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Mitofilin aa 708-758. The exact sequence is proprietary.
Database link: Q16891
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Predicted molecular weight: 84 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Cell proliferation inducing protein 52 antibody
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All lanes : Anti-Mitofilin antibody (ab264372) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysates
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysates
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 30 seconds
Mitofilin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264372 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264372 at 1 μg/ml.
Lane 1: ab264372 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264372 has not yet been referenced specifically in any publications.