Recombinant
RabMAb

Recombinant Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

Overview

  • Product name

    Anti-Mitofilin antibody [EPR8749] - BSA and Azide free
    See all Mitofilin primary antibodies
  • Description

    Rabbit monoclonal [EPR8749] to Mitofilin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Mitofilin aa 50-150.
    Database link: Q16891

  • Positive control

    • Human fetal heart and Human fetal muscle tissue lysates, HeLa, A673, and Saos 2 cell lysates; Human colon and Human testis tissues; HeLa cells
  • General notes

    ab245764 is the carrier-free version of ab137057 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab245764 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Detects a band of approximately 80-84 kDa (predicted molecular weight: 84 kDa).

Target

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing protein 52 antibody
    • Cell proliferation-inducing gene 4/52 protein antibody
    • Heart muscle protein antibody
    • HMP antibody
    • IMMT antibody
    • IMMT_HUMAN antibody
    • Inner membrane protein mitochondrial antibody
    • Inner mitochrondial membrane antibody
    • MICOS complex subunit MIC60 antibody
    • MINOS2 antibody
    • Mitochondrial inner membrane organizing system 2 antibody
    • Mitochondrial inner membrane protein antibody
    • Mitofilin antibody
    • Motor protein antibody
    • p87 antibody
    • p87/89 antibody
    • p89 antibody
    • pig4 antibody
    • PIG52 antibody
    • Proliferation-inducing gene 4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular cancer tissue sections labeling Mitofilin with purified ab137057 at 1/250 dilution (0.508 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Mitofilin with purified ab137057 at 1/100 dilution (1.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Mitofilin with purified ab137057 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Mitofilin with purified ab137057 at 1/250 dilution (0.508 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Immunofluorescent staining of HeLa cells labelling Mitofilin with ab137057 (unpurified) at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab137057) (unpurified) .

  • Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Mitofilin with ab137057 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab137057) (unpurified).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Mitofilin with ab137057 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab137057) (unpurified).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab245764 has not yet been referenced specifically in any publications.

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