Key features and details
- Mouse monoclonal [11E91H12] to Mitofusin 1 (HRP)
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2b
Product nameAnti-Mitofusin 1 antibody [11E91H12] (HRP)
See all Mitofusin 1 primary antibodies
DescriptionMouse monoclonal [11E91H12] to Mitofusin 1 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Recombinant fragment corresponding to Mouse Mitofusin 1.
- WB: NIH 3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesProduced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab198410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
Sequence similaritiesBelongs to the mitofusin family.
modificationsUbiquitinated by MARCH5.
Cellular localizationCytoplasm and Mitochondrion outer membrane.
- Information by UniProt
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
Anti-Mitofusin 1 antibody [11E91H12] (HRP) (ab198410) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 40 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198410 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198410 has not yet been referenced specifically in any publications.