Overview

  • Product name

    Anti-Mitofusin 1 antibody [11E91H12] (HRP)
    See all Mitofusin 1 primary antibodies
  • Description

    Mouse monoclonal [11E91H12] to Mitofusin 1 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Mouse Mitofusin 1.

  • Positive control

    • WB: NIH 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab198410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

Target

  • Function

    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • Tissue specificity

    Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • Sequence similarities

    Belongs to the mitofusin family.
  • Post-translational
    modifications

    Ubiquitinated by MARCH5.
  • Cellular localization

    Cytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • MS996 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

Images

  • Anti-Mitofusin 1 antibody [11E91H12] (HRP) (ab198410) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 40 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 84 kDa


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198410 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab198410 has not yet been referenced specifically in any publications.

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