Product nameAnti-Mitofusin 1 antibody
See all Mitofusin 1 primary antibodies
DescriptionRabbit polyclonal to Mitofusin 1
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
- Human placenta lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab104274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
Sequence similaritiesBelongs to the mitofusin family.
modificationsUbiquitinated by MARCH5.
Cellular localizationCytoplasm and Mitochondrion outer membrane.
- Information by UniProt
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
Anti-Mitofusin 1 antibody (ab104274) at 1 µg/ml + Human placenta lysate at 10 µg
Predicted band size: 84 kDa
All lanes : Anti-Mitofusin 1 antibody (ab104274) at 1/1000 dilution (+ 5% non-fat milk for 16 hours at 4°C )
Lanes 1-2 : Whole tissue lysate of mouse heart (wild type)
Lanes 3-4 : Whole tissue lysate of mouse heart (Knock-out)
Lysates/proteins at 20 µg per lane.
All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 5% Milk for 1 hour at room temperature
This product has been referenced in:
- Klein CP et al. Physical Exercise During Pregnancy Prevents Cognitive Impairment Induced by Amyloid-ß in Adult Offspring Rats. Mol Neurobiol N/A:N/A (2018). Read more (PubMed: 29982984) »
- Mon EE et al. Regulation of mitochondrial iron homeostasis by sideroflexin 2. J Physiol Sci N/A:N/A (2018). Read more (PubMed: 30570704) »