Overview

  • Product name

  • Description

    Rabbit polyclonal to Mitofusin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Mitofusin 1 aa 683-732.
    Sequence:

    QVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQ


    Database link: Q8R4Z9

  • Positive control

    • Human placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab104274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • Tissue specificity

    Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • Sequence similarities

    Belongs to the mitofusin family.
  • Post-translational
    modifications

    Ubiquitinated by MARCH5.
  • Cellular localization

    Cytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • MS996 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

Images

  • Anti-Mitofusin 1 antibody (ab104274) at 1 µg/ml + Human placenta lysate at 10 µg

    Predicted band size: 84 kDa

  • All lanes : Anti-Mitofusin 1 antibody (ab104274) at 1/1000 dilution (+ 5% non-fat milk for 16 hours at 4°C )

    Lanes 1-2 : Whole tissue lysate of mouse heart (wild type)
    Lanes 3-4 : Whole tissue lysate of mouse heart (Knock-out)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking Step: 5% Milk for 1 hour at room temperature

References

This product has been referenced in:

  • Klein CP  et al. Physical Exercise During Pregnancy Prevents Cognitive Impairment Induced by Amyloid-ß in Adult Offspring Rats. Mol Neurobiol N/A:N/A (2018). Read more (PubMed: 29982984) »
  • Mon EE  et al. Regulation of mitochondrial iron homeostasis by sideroflexin 2. J Physiol Sci N/A:N/A (2018). Read more (PubMed: 30570704) »
See all 12 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (Prosate cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Prosate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 11 2017

Application
Western blot
Sample
Pig Tissue lysate - other (Mitochondrial Isolate)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Mitochondrial Isolate
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 22 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
20 µg
Specification
mouse heart
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Oct 11 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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