Product nameAnti-Mitofusin 1 antibody
See all Mitofusin 1 primary antibodies
DescriptionRabbit polyclonal to Mitofusin 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Synthetic peptide corresponding to Human Mitofusin 1 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive in NIH3T3 and MEF1 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab94508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
Sequence similaritiesBelongs to the mitofusin family.
modificationsUbiquitinated by MARCH5.
Cellular localizationCytoplasm and Mitochondrion outer membrane.
- Information by UniProt
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
All lanes : Anti-Mitofusin 1 antibody (ab94508) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 55 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes