Overview

  • Product name

    Anti-Mitofusin 1 antibody [EPR21953-74]
    See all Mitofusin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21953-74] to Mitofusin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse Mitofusin 1 aa 350-450. The exact sequence is proprietary.
    Database link: Q811U4

  • Positive control

    • WB: Mouse brain and heart lysates; Rat brain and heart lysates; NIH/3T3,C2C12 and Neuro-2a whole cell lysates. IP: NIH/3T3 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 84 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
    • Tissue specificity

      Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
    • Sequence similarities

      Belongs to the mitofusin family.
    • Post-translational
      modifications

      Ubiquitinated by MARCH5.
    • Cellular localization

      Cytoplasm and Mitochondrion outer membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Fzo homolog antibody
      • Hfzo1 antibody
      • Hfzo2 antibody
      • MFN 1 antibody
      • Mfn1 antibody
      • MFN1_HUMAN antibody
      • Mitochondrial transmembrane GTPase Fzo 1 antibody
      • Mitochondrial transmembrane GTPase FZO 2 antibody
      • Mitochondrial transmembrane GTPase FZO1B antibody
      • Mitofusin 1 antibody
      • Mitofusin-1 antibody
      • Mitofusin1 antibody
      • MS996 antibody
      • Putative transmembrane GTPase antibody
      • Transmembrane GTPase MFN1 antibody
      see all

    Images

    • Mitofusin 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab221661 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221661 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
      Lane 2: ab221661 IP in NIH/3T3 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221661 in NIH/3T3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

    • All lanes : Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) at 1/1000 dilution

      Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
      Lane 2 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
      Lane 3 : Rat brain lysate
      Lane 4 : Rat heart lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times.

      Lanes 1-3: 3 minutes. Lane 4: 81 seconds.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 25574749).

    • All lanes : Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) at 1/1000 dilution

      Lane 1 : Mouse brain lysate at 20 µg
      Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
      Lane 3 : Mouse heart lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Eposure times.

      Lane 1: 3 minutes. Lanes 2 & 3: 48 seconds.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 25574749).

    References

    ab221661 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab221661.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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