Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21953-74] to Mitofusin 1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Product nameAnti-Mitofusin 1 antibody [EPR21953-74] - BSA and Azide free
See all Mitofusin 1 primary antibodies
DescriptionRabbit monoclonal [EPR21953-74] to Mitofusin 1 - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat
Synthetic peptide within Mouse Mitofusin 1 aa 350-450. The exact sequence is proprietary.
Database link: Q811U4
- IP: NIH/3T3 whole cell lysate.
Ab254198 is the carrier-free version of ab221661. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab254198 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab254198 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
Sequence similaritiesBelongs to the mitofusin family.
modificationsUbiquitinated by MARCH5.
Cellular localizationCytoplasm and Mitochondrion outer membrane.
- Information by UniProt
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
Mitofusin 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab221661 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221661 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221661).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254198 has not yet been referenced specifically in any publications.