Overview

  • Product name

    Anti-Mitofusin 1 antibody [EPR7960]
    See all Mitofusin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7960] to Mitofusin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Mitofusin 1 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat and PC3 cell lysates; Permeabilized Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
    • Tissue specificity

      Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
    • Sequence similarities

      Belongs to the mitofusin family.
    • Post-translational
      modifications

      Ubiquitinated by MARCH5.
    • Cellular localization

      Cytoplasm and Mitochondrion outer membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Fzo homolog antibody
      • Hfzo1 antibody
      • Hfzo2 antibody
      • MFN 1 antibody
      • Mfn1 antibody
      • MFN1_HUMAN antibody
      • Mitochondrial transmembrane GTPase Fzo 1 antibody
      • Mitochondrial transmembrane GTPase FZO 2 antibody
      • Mitochondrial transmembrane GTPase FZO1B antibody
      • Mitofusin 1 antibody
      • Mitofusin-1 antibody
      • Mitofusin1 antibody
      • MS996 antibody
      • Putative transmembrane GTPase antibody
      • Transmembrane GTPase MFN1 antibody
      see all

    Images

    • All lanes : Anti-Mitofusin 1 antibody [EPR7960] (ab129154) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 84 kDa

    • ab129154 at 1/10 dilution staining Mitofusin 1 in permeabilized Jurkat cells by Flow Cytometry (red) or a rabbit IgG negative control (green).

    References

    This product has been referenced in:

    • Yi X  et al. SIRT3-Dependent Mitochondrial Dynamics Remodeling Contributes to Oxidative Stress-Induced Melanocyte Degeneration in Vitiligo. Theranostics 9:1614-1633 (2019). Read more (PubMed: 31037127) »
    • Govender J  et al. Melatonin improves cardiac and mitochondrial function during doxorubicin-induced cardiotoxicity: A possible role for peroxisome proliferator-activated receptor gamma coactivator 1-alpha and sirtuin activity? Toxicol Appl Pharmacol 358:86-101 (2018). Read more (PubMed: 29966675) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Q&A

    Answer

    I believe there has been a misunderstanding here.

    We try to provide all the information which we have regarding any product we carry and provide the most thorough data we can on our datasheets as you have undoubtably seen when using our Mitofusin 2 antibody. Our products made in house will invariably have that information available as well. We do have over 70,000 products on our catalog and this data is available to many of those products. However, we also receive a number of products from outside suppliers. Some of these products will have licensing agreements which preclude providing detailed immunogen sequences. In these instances we try and provide a range within immunogen protein against which the antibody is made.

    I hope this information helps you to understand our previous communication. Please let me know if you have any further questions or concerns.

    Read More

    Answer

    Thank you very much for your interest in our Mitofusin 1 antibodies.

    Unfortunately the exact sequences of many of the immunogens used to create our proprietary. I have BLAST-ed the human and mouse Mitofusin 1 sequences and found that the identities between those species and dog were 91% and 94% respectively.

    To our knowledge, these products have not been tested in dog tissues. Therefore, I can offer a discount off a future purchase if you purchase now, test it in dog tissue and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    As you are interested in WB and IHC-P may I recommend one of the following products as they have both been shown to work in those applications?

    https://www.abcam.com/Mitofusin-1-antibody-ab104585.html

    https://www.abcam.com/Mitofusin-1-antibody-ab57602.html


    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know which antibody against Mitofusin 1 you would like to proceed and test. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

    2. Purchase either by phone, fax, or online (www.abcam.com).

    3. Test it in dog tissue.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody) turns out to be unsuitable for this species, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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