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Previously we used your chicken polyclonal to MFN1 (ab60939) to good effect on dog tissue for WB and IHC-P(Fyfe JC et al Neurogentics 2011;12:223-232, (fig 1 and 2). Now when we need it again, I find you do not have it. Since we are working in dog, I need to know the immunogen peptide sequence so that I can gauge how similar the dog is to the human sequence used. Your current ab 129154 looks the most likely, but ab104274 may also be suitable.
Asked on Apr 03 2012
Thank you very much for your interest in our Mitofusin 1 antibodies.
Unfortunately the exact sequences of many of the immunogens used to create our proprietary. I have BLAST-ed the human and mouse Mitofusin 1 sequences and found that the identities between those species and dog were 91% and 94% respectively.
To our knowledge, these products have not been tested in dog tissues. Therefore, I can offer a discount off a future purchase if you purchase now, test it in dog tissue and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
As you are interested in WB and IHC-P may I recommend one of the following products as they have both been shown to work in those applications?
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know which antibody against Mitofusin 1 you would like to proceed and test. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.
2. Purchase either by phone, fax, or online (www.abcam.com).
3. Test it in dog tissue.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody) turns out to be unsuitable for this species, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Answered on Apr 03 2012