Anti-Mitofusin 1 antibody - N-terminal (ab191853)
Key features and details
- Rabbit polyclonal to Mitofusin 1 - N-terminal
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mitofusin 1 antibody - N-terminal
See all Mitofusin 1 primary antibodies -
Description
Rabbit polyclonal to Mitofusin 1 - N-terminal -
Host species
Rabbit -
Specificity
ab191853 is predicted to not cross-react with Mitofusin 2. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Mitofusin 1 (N terminal). The exact sequence is proprietary. Peptide corresponds to 17 amino acids near the amino terminus (NP_284941).
Database link: Q8IWA4 -
Positive control
- A431 cells and cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 84 kDa).
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Notes |
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ICC
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 84 kDa). |
Target
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Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. -
Tissue specificity
Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. -
Sequence similarities
Belongs to the mitofusin family. -
Post-translational
modificationsUbiquitinated by MARCH5. -
Cellular localization
Cytoplasm and Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 55669 Human
- Omim: 608506 Human
- SwissProt: Q8IWA4 Human
- Unigene: 478383 Human
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Alternative names
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
see all
Images
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Anti-Mitofusin 1 antibody - N-terminal (ab191853) at 1 µg/ml + A431 cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted? -
Immunocytochemistry analysis of A431 cells, labeling Mitofusin 1 using ab191853 at 5 μg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab191853 has been referenced in 1 publication.
- Kshirsagar S et al. A Combination Therapy of Urolithin A+EGCG Has Stronger Protective Effects than Single Drug Urolithin A in a Humanized Amyloid Beta Knockin Mice for Late-Onset Alzheimer's Disease. Cells 11:N/A (2022). PubMed: 36078067