Key features and details
- Rabbit polyclonal to Mitofusin 1 - N-terminal
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Mitofusin 1 antibody - N-terminal
See all Mitofusin 1 primary antibodies
DescriptionRabbit polyclonal to Mitofusin 1 - N-terminal
Specificityab191853 is predicted to not cross-react with Mitofusin 2.
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Mitofusin 1 (N terminal). The exact sequence is proprietary. Peptide corresponds to 17 amino acids near the amino terminus (NP_284941).
Database link: Q8IWA4
- A431 cells and cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 84 kDa).|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
Sequence similaritiesBelongs to the mitofusin family.
modificationsUbiquitinated by MARCH5.
Cellular localizationCytoplasm and Mitochondrion outer membrane.
- Information by UniProt
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
Anti-Mitofusin 1 antibody - N-terminal (ab191853) at 1 µg/ml + A431 cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Immunocytochemistry analysis of A431 cells, labeling Mitofusin 1 using ab191853 at 5 μg/mL.
ab191853 has not yet been referenced specifically in any publications.