Western blot abreview for Anti-Mitofusin 2 antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Larval lysate)
Loading amount
50 µg
Specification
Larval lysate
Treatment
WT, MFN knockdown, and Pink1 mutant lysates were tested
Gel Running Conditions
Reduced Denaturing (9% GEL)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: NA931V ecl anti-mouse IgG HRP
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Millipore ECL
Exposure
20 minute(s) and 0 second(s)
Positive control
WT and Pink1 mutant the latter upregules mfn in vivo
Negative control
Knockdown using RNAi of protein

Additional data

Abcam response
Thank you for your feedback. This customer was given a testing discount to trial this antibody in drosophila, an untested species. These preliminary data suggest that this antibody may not be suitable for drosophila, but further testing is necessary.

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Submitted Jul 09 2012

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