Western blot abreview for Anti-Mitofusin 2 antibody

Good
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk with 0.1% Triton X100
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 80 kDa Non-specific: 28, 55 kDa
Exposure
1 minute(s) and 0 second(s)

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Submitted Jun 09 2014

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